Indirect Immunofluorescence Assays (IFA)
The Gold Standard in Autoimmune Screening.
The Indirect Immunofluorescence assays (IFA) play a key role in the detection and measurement of autoantibodies. They combine immunological principles with fluorescence microscopy to provide highly specific and sensitive results. Indirect immunofluorescence is commonly used to detect autoantibodies in patients with conditions such as systemic lupus erythematosus (SLE) and Sjögren’s disease. It's considered the gold standard for the detection of antinuclear antibodies (ANA) in patient's with suspected Systemic Autoimmune Disorder (SARD).
About Indirect Immunofluorescence Assays (IFA)
In an Indirect Immunofluorescence assay, patient serum is incubated with substrate-bound antigens on a slide. If specific autoantibodies are present, they bind to the antigens. Fluorescently labeled secondary antibodies are added, which attach to the autoantibodies.
Under a fluorescence microscope, the secondary antibodies emit light when exposed to specific wavelengths. The resulting patterns reveal the presence of autoantibodies and aid disease diagnostics.
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Knowledge & Science
This technical whitepaper outlines the use of the Indirect Immunofluorescence technique on HEp-2 cells as the gold standard for Antinuclear Antibodies screening.
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